Figure 4

Cell invasion and clonogenic assays. (A) Matrigel invasion activity of CD24+/CD44+ and CD24-/CD44+ flow cytometry-sorted cells from HNSCC cell lines. The number of cells invading through the Matrigel was assessed at 24 hr. (B) Colony-forming assay with FACS-sorted CD24+/CD44+ and CD24-/CD44+ cells. The CD24+/CD44+ cells show significantly higher number of colonies. P values for invasion and clonogenic assays are shown in the figure.