| 1stRun | 2ndRun |
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Evaluation | Kappa1
| % Agreement | Kappa | % Agreement |
Positive v Negative2
| 0.94 | 97.2 | 0.98 | 98.9 |
Staining intensity3
| 0.59 | 75.0 | 0.81 | 87.9 |
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1Kappa values where calculated as described in Methods where the agreement between operators is defined as follows: < 0 none, 0–0.20 slight, 0.21–0.40 fair, 0.41–0.60 moderate, 0.61–0.80 substantial, and 0.81–1 almost perfect [22].
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2This table entry refers to an overall assessment of the level of positive versus negative staining regardless of the staining intensity.
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3Staining intensity was graded into 4 categories as negative, weak (1 +), moderate (2 ++) or strong (3 +++).