Table 1 Primer sequences used for RT-PCR
From: The novel IGF-IR/Akt–dependent anticancer activities of glucosamine
Gene | Primer sequence (5′ - 3′) |
|---|---|
IGF-1R | F 5′-ACG CCA ATA AGT TCG TCC AC-3′ |
R 5′-TCC ATC CTT GAG GGA CTC AG-3′ | |
COX-2 | F 5′-ATC TTT GGG GAG ACC ATG GTA GA-3′ |
R 5′-ACT GAA TTG AGG CAG TGT TGA TG-3′ | |
XBP1 | F 5′-TTA CGA GAG AAA ACT CAT GGC C-3′ |
R 5′-GGG TCC AAG TTG TCC AGA ATG C-3′ | |
GRP78 | F 5′-GGT ACA TTT GAT CTG ACT G-3′ |
R 5′-CAC TTC ACT AGA GTT TGC TG-3′ | |
CHOP | F 5′-CTT CAC TAC TCT TGA CCC TGC AT-3′ |
R 5′-ATG TGC ACT GGA GAT TGC TT-3′ | |
ATF4 | F 5′-GTT CTC CAG CGA CAA GGC TA-3′ |
R 5′-ATC CTC CTT GCT GTT GTT GG-3′ | |
GAPDH | F 5′-GGT GAA GGT CGG TGT GAA CGG ATT T-3′ |
R 5′-ATT GCC AAA GTT GTC ATG GAT GAC C-3′ | |
β-ACTIN | F 5′-GTG GGG CGC CCC AGG CAC CA-3′ |
R 5′-CTC CTT AAT GTC ACG CAC GAT TTC-3′ |