Table 1 Details of the immunohistochemical procedure used to analyze the expression of the different proteins
Protein | Antibody | Company | Antibody dilution | Positive control | Incubation period | Detection system |
|---|---|---|---|---|---|---|
MCT4 | sc-50329 | Santa Cruz Biotechnology | 1:500 | Colon tumor | Overnight | R.T.U. Vectastain Universal Elite ABC Kit, Vector, EUA |
GLUT1 | ab 15309 | Abcam | 1:2000 | Head and neck tumor | 2 hours | Ultravision Detection System Anti-polyvalent, HRP, Labvision Corporation, Freemont, CA |
CAIX | ab 15086 | Abcam | 1:2000 | Stomach | ||
MCT1 | sc-365501 | Santa Cruz Biotechnology | 1:500 | Colon tumor | Overnight | R.T.U. Vectastain Universal Elite ABC Kit, Vector, EUA |
GLUT12 | ab 75441 | Abcam | 1:500 | Kidney | Overnight | |
LDHV | ab 53010 | Abcam | 1:1000 | Colon tumor | ||
PDK1 | ab 110025 | Abcam | 1:500 | Stomach | ||
AMACR | 504R-16 | Cell Marque | 1:50 | Kidney | ||
ACOX3 | sc-135435 | Santa Cruz Biotechnology | 1:250 | Liver | 2 hours | Ultravision Detection System Anti-polyvalent, HRP, Labvision Corporation, Freemont, CA |
DBP | DBP antibody was a gift from Dr. Gabriele Moller from HelmholtzZentrum mÜnchen | Ready to use | Kidney | |||