Table 2 Summary of differential protein spots of urine between patients with UTUC and healthy people identified by LC-MS/MS

1

ATP-binding cassette sub-family A member 9

Q8IUA7

184.2/6.49

10

1

38

2

sorting nexin-14

Q9Y5W7

102.8/6.4

17

3

34

3

serum albumin precursor

P02768

69.3/5.92

1

2

57

4

F-box/WD repeat protein 1A

Q9Y297

68.8/8.3

29

3

33

5

spermatogenesis-associated protein 7

Q9P0W8

67.6/5.9

3

4

34

6

actin

P62736

41.9/5.23

2

9

45

7

cytokeratin 1

P04264

65.9/8.16

3

3

40

8

DNA replication licensing factor MCM2

P49736

101.8/5.34

40

1

50

9

proline-serine-threonine phosphatase-interacting protein 1

O43586

47.5/5.53

6

4

40

10

X box-binding protein 1

P17861

28.6/9.71

18

3

35

11

immunoglobulin kappa chain C region

P01834

11.6/5.58

16

49

151

12

immunoglobulin kappa chain V-I region AG

P01593

11.9/5.67

4

31

176

13

hemopexin precursor

P02790

51.6/6.55

4

7

86

14

prostaglandin-H2 D-isomerase precursor

P41222

21.0/7.66

3

12

33

15

immunoglobulin kappa chain C region

P01834

11.6/5.58

22

49

217

16

gelsolin precursor

P06396

85.6/5.9

9

7

208

17

SH3-containing GRB2-like protein 3

Q99963

39.2/5.27

16

8

32

18

hemoglobin subunit beta

P68871

15.9/6.75

9

32

214

19

inter-alpha-trypsin inhibitor heavy chain H4

Q14624

103.2/6.51

13

10

243

20

cytokeratin 10

P13645

59.4/5.13

2

4

38

21

basement membrane-specific heparan sulfate proteoglycan core protein precursor

P98120

468.8/6.06

116

4

2114

22

retinol Binding Protein 4

P02753

22.9/5.76

45

35

233

23

Zn-alpha2-glycoprotein

P25311

34.7/5.71

14

45

378

24

Zn-alpha2-glycoprotein

P25311

34.7/5.71

5

16

98

25

fibrinogen gamma chain precursor

P02679

51.4/5.37

19

24

201

26

fibrinogen gamma chain precursor

P02679

51.4/5.37

5

11

38

27

serotransferrin precursor

P02787

77.0/6.81

7

5

48

28

serotransferrin precursor

P02787

77.0/6.81

18

13

88

29

annexin A3

P12429

36.3/5.63

3

4

37

30

dermcidin precursor

P81605

11.2/6.08

2

16

48

31

calreticulin

P27797

48.1/4.29

19

36

493

32

haptoglobin

P00738

45.1/6.13

39

13

432

33

haptoglobin

P00738

45.1/6.13

41

20

466

34

haptoglobin

P00738

45.1/6.13

49

17

668

35

haptoglobin

P00738

45.1/6.13

19

15

271

36

haptoglobin

P00738

45.1/6.13

5

13

77

37

glial fibrillary acidic protein

P14136

49.8/5.42

2

4

43

38

alpha-1-microglobulin/bikunin precursor

P02760

38.9/5.95

41

14

698

39

alpha-1-microglobulin/bikunin precursor

P02760

38.9/5.95

48

15

732

Spot no

Protein name

Accession no

Calculate Mw/ pI

Peptide matched

Sequence covered%

MASCOT score

40

alpha-1-microglobulin/bikunin precursor

P02760

38.9/5.95

45

23

667

41

syntaxin-binding protein 4

Q6ZWJ1

61.7/5.16

4

7

40

42

mRNA cap guanine-N7 methyltransferase

O43148

54.8/6.3

11

3

52

43

transthyretin precursor

P02766

15.8/5.52

13

48

217

44

transthyretin precursor

P02766

15.8/5.52

8

40

142

45

alpha-1-microglobulin/bikunin precursor

P02760

38.9/5.95

5

11

63

46

alpha-1-microglobulin/bikunin precursor

P02760

38.9/5.95

13

11

198

47

transthyretin precursor

P02766

15.8/5.52

4

14

46

48

serine/threonine-protein kinase MRCK beta

Q9Y5S2

194.1/5.91

3

2

56

49

serum albumin precursor

P02768

69.3/5.92

51

25

594

50

serotransferrin precursor

P02787

77.0/6.81

42

21

441

51

serum albumin precursor

P02768

69.3/5.92

92

26

1359

52

serum albumin precursor

P02768

69.3/5.92

48

26

662

53

annexin A2

P07355

38.58/7.57

28

37

505

54

Ig gamma-1 chain C region

P01857

36.0/8.46

20

26

190

55

Ig gamma-1 chain C region

P01857

36.0/8.46

12

25

104