Figure 4From: Docosahexaenoic acid-induced apoptosis is mediated by activation of mitogen-activated protein kinases in human cancer cells Activation of MAPKs is responsible for the apoptosis induced by DHA. (A) Indicated MAPKs inhibitors were added to H1299 cells 1Â h before DHA treatment for 24Â h. The protein levels of PARP and MAPKs were then examined by western blot. (B) Indicated cancer cells were treated with non-targeting control siRNA (siNC) or siRNAs specific for conventional MAPKs genes (siERK, siJNK and sip38). At 18Â h after transfection, cells were incubated with the indicated doses of DHA for 24Â h (12Â h in cases of PA-1 cells). Then, cells were harvested and western analysis was performed using the following antibodies: PARP, MAPKs and actin. The data shown are representative of three independent experiments with similar results.Back to article page