Figure 5

Regulation of PLK2 in the proliferation and apoptosis in the Hep2 cells. (A) Relative PLK2 mRNA levels in the Hep2 cells. Hep2 cells were silenced by siRNA-PLK2 and the PLK2 mRNA was assayed by qRT-PCR. GAPDH was used as internal control. (B) Relative PLK2 protein levels in the Hep2 cells. Hep2 cells were silenced by siRNA-PLK2 and the PLK2 protein was detected by Western blot. β-actin was used as internal control. (C) Effect of siRNA-PLK2 on the Hep2 cell proliferation measured by the MTT assay. Hep2 cells were transfected with siRNA-PLK2 or the control miRNAs in the Hep2 cells and the cell proliferation was detected using the MTT assay. (D) Effect of siRNA-PLK2 on the Hep2 cell proliferation measured by the colony formation assay. Hep2 cells were transfected with siRNA-PLK2 or the control miRNAs in the Hep2 cells and the cell proliferation was detected using the colony formation assay. (E) Effect of siRNA-PLK2 on the late apoptosis of the Hep2 cell lines. Hep2 cells were transfected with siRNA-PLK2 or the control miRNAs and treated by Annexin V-EGFP apoptosis detection kit. The late apoptotic percentages of the Hep2 cells in different groups were monitored by flow cytometry. Data were expressed as the mean ± SD from three independent experiments. P < 0.05 is indicated as symbol*.