Figure 4

Effect of resveratrol on the survival of the parental HCT116 cells and the bax -/- derivatives in culture. Panel A: Treatment of exponentially growing cultures with different doses of resveratrol for 48 h and quantitation of the numbers of cells with a sub-2n-DNA content by flow-cytometry revealed a dose-dependent increase of apoptosis in both cell lines and a much stronger apoptotic response in the cells expressing Bax, especially at low drug doses. Panel B: Time course of apoptosis in exponentially growing cultures either mock-treated or treated with 100 μM resveratrol, documenting the time-dependence of cell death induction. Note the reduced basal level of apoptosis in the bax -/- cultures. Error bars denote standard deviations of the means of four experiments.