Figure 3

Cytotoxic effect of gemcitabine-loaded liposomes. ARO cells were grown to 50% confluence and treated with increasing concentrations of either free gemcitabine or gemcitabine-loaded liposomes for 24 h. Cell mortality was evaluated by trypan bleu dye exclusion assay (see Methods). The values, expressed as the ratio over control, represent the mean ± SEM of at least three different experiments in triplicates.