Figure 1

Cytokine response of lung cells. A. Primary cultures of the normal type II epithelial cells and fibroblasts from residual lung tissue and the indicated cell lines were treated for 15 min with factors listed at the bottom. Extracts were analyzed by immunoblotting for phosphorylated STAT3 and ERK-1/-2. The level of immunodetectable total ERK-1/-2 indicates protein loading. B. The quantitative values (mean and SD) for the phosphorylation of ERK-1/-2 (open bars) and STAT3 (closed bars) by treatments with LIF, OSM, IL-6 and EGF were determined in 5 independent experiments (expressed relative to the OSM effect, equal 100%). C. Extracts from control treated cultures from A were analyzed for the immunodetectable level of total STAT3 and ERK-1/-2. D. Type II epithelial cells were treated for the indicated length of time with OSM (100 ng/ml) and the level of phosphorylated STAT3 and ERK-1/-2 was determined by immunoblotting.