A phase I study of hydralazine to demethylate and reactivate the expression of tumor suppressor genes

BMC Cancer

Table 2 Primers and conditions used for RT-PCR

Primer set	Sense 5'-- 3'	Antisense 5'--- 3'	Size	Annealing
p16	AGCCTTCGGCTGACTGGCTGG	CTGCCCATCATCATGACCTGG	150	60°C
RARβ	GACTGTATGGATGTTCTGTCAG	ATTTGTCCTGGCAGACGAAGCA	146	50°C
MGMT	GCTGCAGACCAVCTCTGTGGCACG	GCCGCCTCTTCACCATCCCG	81	50°C
FHIT	ATGTCGTTCAGATTTGGCCAAC	TCATAGATGCTGTCATTCCTGT	340	53°C
DAPK	AACCCATCATCCATGCCATC	TCTCTCCTTCTCGGTTCTTGA	200	51°C
APC	GAGACAGAATGGAGGTGCTGC	GTAAGATGATTGGAATTATCTTCT	180	56°C
GSTp1	TCCGCTGCAAATACATCTCC	TGTTTCCCGTTGCCATTGAT	320	50°C
ER	GGAGACATGAGAGCTGCCAAC	CCAGCAGCATGTCGAAGATC	480	55°C

ISSN: 1471-2407