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Table 3 Polyamine levels, enzymatic activities and genes expression in neoplastic colorectal tissue with and without K-ras mutation.

From: Peroxisome proliferator-activated receptor gamma and spermidine/spermine N1-acetyltransferase gene expressions are significantly correlated in human colorectal cancer

 

Mutated K-ras n = 12

Non mutated K-ras n = 28

p

Putrescine

64.4 ± 14.7

41.0 ± 14.5

0.039

Spermidine

376.1 ± 49.2

248.8 ± 26.9

0.021

Spermine

652.2 ± 48.6

455.5 ± 55.99

0.020

Total polyamines

1093.0 ± 98.0

745.3 ± 85.5

0.012

ODC

525.1 ± 157.6

92.9 ± 26.5

0.002

SSAT

43.1 ± 5.6

25.92 ± 4.3

0.035

ODC mRNA

21.3 ± 4.4

8.79 ± 1.8

0.020

SSAT mRNA

18.0 ± 3.9

7.3 ± 1.4

0.011

PPARγ mRNA

2.9 ± 1.1

0.78 ± 0.2

0.032

  1. Polyamines are reported as nmol/g wet tissue. ODC activity is expressed as pmol 14CO2/h/mg of protein. SSAT activity is expressed as pmol N1-[14C] acetylspermidine/min/mg of protein. The relative mRNA levels are expressed as target gene/β-actin ratio. All data are presented as mean ± SEM (Mann Whitney test).
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BMC Cancer

ISSN: 1471-2407

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