Figure 3
From: Nestin expression in the cell lines derived from glioblastoma multiforme
Arrangement of nestin filaments and microtubules in the same cell. Double labeling for both nestin (A) and tubulin (B) in the same cell of GM10 cell line showed no co-localization of nestin filaments with microtubules and confirmed different morphology of these cytoskeletal structures. Nestin-positive filaments (A, green) stained by indirect immunofluorescence using FITC-labeled secondary antibody; microtubules (B, red) stained by the same method using TRITC-labeled secondary antibody; nucleus labeled by DAPI (C, blue) are shown; bar, 10 μm.