Table 2 Primers and Probes for real-time PCR
From: Favorable prognostic value of SOCS2 and IGF-I in breast cancer
Gene Symbol | Sequence | |
|---|---|---|
SOCS1 | Forward | 5'-TTTTCGCCCTTAGCGTGAAG-3' |
Reverse | 5'-CATCCAGGTGAAAGCGGC-3' | |
Taqman probe | 5'FAM-CCTCGGGACCCACGAGCATCC-3'TAM | |
SOSC2 | Forward | 5'-CAGATGTGCAAGGATAAGCGG-3' |
Reverse | 5'-CAGATAAAGGTGAACAGTGCCG-3' | |
Taqman probe | 5'FAM-CAGGTCCAGAAGCCCCCCGG-3'TAM | |
SOCS3 | Forward | 5'-TGATCCGCGACAGCTCG-3' |
Reverse | 5'-TCCCAGACTGGGTCTTGACG-3' | |
Taqman probe | 5'FAM-CCAGCGCCACTTCTTCACGCTCA-3'TAM | |
CIS | Forward | 5'-TCCAACTGCTTGTCCAGGC-3' |
Reverse | 5'-GTGCTGCACAAGGCTGACC-3' | |
Taqman probe | 5'FAM-ACGCATCCTGGCCTTTCCGGA-3'TAM | |
TBP | Forward | 5'-CACGAACCACGGCACTGATT-3' |
Reverse | 5'-TTTTCTTGCTGCCAGTCTGGAC-3' | |
Taqman probe | 5'FAM-TCTTCACTCTTGGCTCCTGTGCACA-3'TAM | |
IGF-I | Forward | 5'-TCAGCTCGCTCTGTCCGTG-3' |
Reverse | 5'-TGACTCCCTCTACTTGCGTT-3' | |
Taqman probe | 5'FAM-TGCCCAAGACCCAGAAGGAAGTACATTTG-3'TAM |