Figure 2
From: HIF2α reduces growth rate but promotes angiogenesis in a mouse model of neuroblastoma
HIF2α induces neuroblastoma cell hypertrophy. a) Morphology of control N1E-115 (NT), and N1E-115 transfected with HIF2α and HIF2α(1–485) vectors. b) RT-PCR analyses of neural crest differentiation markers (Id2 and CgA) c) Quantification of the average cell diameter of each clone. Cell size data are mean ± SEM of 100 values for each clone, reported to 100% for NT cells. d) The difference in cell size was not attributable to modification in neither Akt protein expression nor phosphorylation as revealed by Western blot performed on protein extracted from the different clones.