Figure 2

Down-regulation of telomerase activity by 5-Fu. (A) Telomerase activity assay. SMMC-7721 cells were harvested after incubation with 62.5 μg/ml of 5-Fu for 36 h. The telomerase activity was measured by TRAP-silver staining assay. (Lanes 1–4: 293 cell lysate as positive control, heat-treated (65°C, 10 min) SMMC-7721 cell lysate as negative control, SMMC-7721 cell lysate not treated with 5-Fu, SMMC-7721 cell lysate treated with 5-Fu (62.5 μg/ml) for 36 h,respectively). (B) hTERT mRNA expression determined with RT-PCR.