Figure 9

Image Analysis of bladder cross-sections obtained at 120, 330, and 1440 minutes (n = 3 mice per time point). Six random fields per cross-section were visualized at 20× magnification and used for image analysis that was performed with the NIS-Elements Advanced Research 2.3 imaging software (see Methods). Results are presented as mean and SEM. Asterisks indicate a statistically significant difference (p < 0.05) between the bladder area covered by blood vessels (CD-31) and lymphatic vessels (LYVE-1), whereas a pound sign indicates a statistically significant difference (p < 0.05) between the distribution of Cy5.5 in CD-31-positive blood vessels and LYVE-1-positive lymphatic vessels.