Figure 5

Histology of KCI-18 kidney tumors treated with genistein and radiation. Kidney tumors, resected from mice of the experiment described in Figure 4, were processed for histology and tumor sections were stained with H&E. The main findings were labeled on the prints with Tfor tumor, Vfor vessel, Mfor mitosis, Hfor hemorrhages, DGfor degenerative, Nfor necrosis, Afor apoptosis, Ffor fibrosis, DCfor detached cells, Rfor rhabdoid cells, NKTfor normal kidney tissue, IFfor inflammatory cells and AMfor abnormal mitosis. Panels A, B: Kidney tumor from control mice showing high-grade and very vascularized carcinoma (A, ×50) with frequent mitosis (B, ×100). Panels C, D: Kidney tumor from mice treated with genistein showing extensive hemorrhages (C, ×50), degenerative changes in tumor cells, apoptotic cells and areas of necrosis (D, ×100). Panels E, F: Irradiated kidney tumor, with areas of tumor destruction, showing fibrosis and apoptotic cells (E, ×50), focal areas with atypical detached rhabdoid cells (F, ×100). Panels G, H: Kidney tumor from mice treated with genistein and radiation, showing smaller residual tumor area adjacent to normal kidney tissue (G, ×25). The residual tumor looked hemorrhagic and consisted of large areas of detached rhabdoid cells, atypical giant cells with large nuclei and inflammatory cells (H, ×100). Lower and higher magnifications (×-fold) are presented to both show wider areas of tumor histology and focus on major findings.