Figure 4From: Silencing of the XAF1 gene by promoter hypermethylation in cancer cells and reactivation to TRAIL-sensitization by IFN-β Stable XAF1 knockout cell lines are resistant to IFN-β-mediated XAF1 induction. The parental SF539 cell line and 3 stable xaf1 shRNA clones (SFX1, SFX3, SFX4) were exposed to 0 or 500 U/mL IFN-β for 24 hours. Total protein extracts were resolved by 12% SDS-PAGE and sequentially probed with XAF1, XIAP and GAPDH antibodies.Back to article page