Figure 2

FISH analysis on metaphases from bone marrow slides. (A) FISH with the dual color, dual fusion BCR/ABL1 probe generates two fusion signals (ABL1 red and BCR green): one on a marker chromosome [der(17)] and one on the Ph-chromosome, one red signal on a normal chromosome 9 and one green signal on a normal chromosome 22. (B) EVI1 specific probes RP11-362K14 (red, distal from EVI1) and RP11-82C9 (green, EVI1 overlapping) showed a co-localized red-green signal on the normal chromosome 3, a green signal on the derivative chromosome 3 [der(3)] and two red signals translocated onto identical but unidentified chromosomes [der(9)]. (C) FISH with WCPs for chromosomes 3 (purple), 9 (red) and 22 (green), revealed one normal chromosome 9, two derivative chromosomes 9 [der(9)], a der(17) containing a piece of chromosome 22 and chromosome 9, a normal chromosome 3 (norm 3), a derivative chromosome 3 [der(3)] and a Ph-chromosome. (D) WCP of chromosomes 17 (green) and 22 (red), combined with a purple centromeric probe for chromosome 3, indicated that a translocation between the long arm of chromosome 3 and the long arm of chromosome 17 had occurred as well as a translocation between chromosome 17 and 22.