Figure 3

BCL9 and B9L are Wnt-inducible genes. A, Transcript levels of BCL9 and B9L in comparison to AXIN2, as measured by RT-qPCR, after induction of Wnt pathway activity in HEK 293 cells by addition of Wnt3A-conditioned medium, or 20 mM LiCl, for 6 hours. Statistical significance (p < 0.01) relative to Wnt-stimulated cells (arbitrarily set to 100) is indicated by asterisks. B and C, Transcript levels of c-myc and AXIN2 in Wnt-stimulated HEK 293 cells, measured by RT-qPCR as in A, after overexpression of wt or mutant FLAG-BCL9, or FLAG-BCL9-2. Statistical significance relative to Wnt-stimulated cells (arbitrarily set to 100) is indicated by asterisks (*, p < 0.05; **, p < 0.005). D, Transcript levels of BCL9, B9L, AXIN2, c-myc and CD44 relative to TBP (as internal control) in HEK 293 (with or without Wnt stimulation), SW480 or HCT116 cells. Statistical significance relative to uninduced HEK 293 cells is indicated by asterisks (*, p < 0.01; **, p < 0.001).