DNA methylation patterns in bladder cancer and washing cell sediments: a perspective for tumor recurrence detection

Table 1 Oligonucleotide sequences, annealing temperatures, and product size for MSP analysis.

Gene (MS)	Primers 5'- 3'	Position of interrogated CpGs*	Ta (°C)	Product size (bp)	Ref.
*CDH1*
Methylated allele	GTAGTTACGTATTTATTTTTAGTGGCGTC (F)	-14; 4; 7	53	112	[43]
	CGAATACGT CGAATCGAACCG (R)	68; 73; 78; 82; 88
Unmethylated allele	TGGTTGTAGTTATGTATTTATTTTTAGTGGTGTT (F)		53	120
	ACACCAATACAACAAATCAAACCAAA (R)
*RARB*
Methylated allele	GAACGCGAGCGATTCGAGT (F)	111; 113; 117; 122	55	158	[44]
	GACCAATCCAACCGAAACG (R)	231; 236; 249
Unmethylated allele	GGATTGGGATGTTGAGAATG (F)		55	143
	CAACCAATCCAACCAAAACAA (R)
*SFN*
Methylated allele	GGTAGTTTTTATGAAAGGCGTC (F)	153; 156	56	106	[33]
	CCTCTAACCGCCCACCACG (R)	219; 228
Unmethylated allele	ATGGTAGTTTTTATGAAAGGTGTT (F)		56	104
	CCCTCTAACCACCCACCACA (R)
*RASSF1A*
Methylated allele	GGGTTTTGCGAGAGCGCG (F)	-66; -60; -58	55	169	[45]
	GCTAACAAACGCGAACCG (R)	77; 82; 84; 94
Unmethylated allele	GGTTTTGTGAGAGTGTGTTTAG (F)		55	169
	CACTAACAAACACAAACCAAAC (R)

MSP – Methylation-Specific Polymerase Chain Reaction; MS – methylation status; * in relation to transcription start site; Ta – Annealing temperature; bp – base pair; F – forward primer; R – reverse primer.

ISSN: 1471-2407