Figure 4

GFAP and vimentin immunocytochemistry through cell subcultivation. Notice the negative modulatory expression pattern to GFAP (A – D) concomitant to the positive modulatory pattern to vimentin (E-H) in NG97(ht). The GFAP appears intensely marked in 100% of the 19^th cell passage cytoplasm (A), being presented in approximately 85% of the 30^th (B) and, finally, totally absent in 51^st (C) and 80^th (D) cell passages. Nonetheless, the vimentin were absent at 21^st (E) cell passage, presented in weakly areas in the 40^th (F) and in 54% of the 59^th (G) cell passage and, finally, markedly positive at 83^rd (H) cell passage (Original magnification: A – C and E – G = 200× and D and H = 1000×) (Positive and negative controls were assayed but were not displayed here).