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Table 1 EGFR primers

From: Genomic activation of the EGFR and HER2-neu genes in a significant proportion of invasive epithelial ovarian cancers

 

First step (5'—3')

Second step (5'—3')

Product Size(BP)

Exon18

F: agcatggtgagggctgag

F: *gctgaggtgacccttgtctc

258

 

R: acagcttgcaaggactctgg

R: acagcttgcaaggactctgg

 

Exon19

F: catgtggcaccatctcaca

F: catgtggcaccatctcaca

179

 

R: ccacacagcaaagcagaaac

R: *ggtgtgtcgtttcgtctttg

 

Exon20

F: cgaagccacactgacgtg

F: cgaagccacactgacgtg

244

 

R: ctatcccaggagcgcagac

R: *ccgtatctcccttccctgat

 

Exon21

F: cctcacagcagggtcttctc

F: cctcacagcagggtcttctc

215

 

R: aatgctggctgacctaaagc

R: *ccgactggatttcg

 

Exon22

F: tttttccaacagagggaaact

F: *cactgcctcatctctcacca

237

 

R:aaagaaaatacttgcatgtcagagg

R:aaagaaaatacttgcatgtcagagg

 

Exon23

F: ccactgccttcttttcttgc

F: *tttcttgcttcatcctctcag

205

 

R: cagctaggcagtgtggacag

R: cagctaggcagtgtggacag

 

Exon24

F: gcatcaccaatgccttcttt

F: *gcaatgccatctttatcatttc

200

 

R: actcttcccaatggaagcac

R: actcttcccaatggaagcac

 
  1. EGFR primers used for amplification (hemi-nested PCRs).
  2. * indicates that a GC clamp was added at the 5' end of the primer,
  3. GC clamp: cgcccgccgcgccccgcgcccggcccgccgcccccgcccg