Figure 1
From: Leptin/HER2 crosstalk in breast cancer: in vitro study and preliminary in vivoanalysis
Expression of HER2 and ObR in breast cancer cell lines. The expression of HER2 (185 kDa) and different isoforms of ObR (150–190 kDa, indicated by arrows) was detected in 50 μg of total protein lysates obtained from MCF-7, BT-474, SK-BR-3, and ZR-75 cell lines by WB with specific Abs, as described in Materials and Methods. The presence of a constitutively expressed enzyme GAPDH was assessed in the same blot as control of protein loading.