Figure 1

Unsupervised hierarchical cluster analysis of the 19 primary tumor cell cultures without doxorubin treatment. Gene signal intensities were normalized to the mean signal of all samples, log2 transformed and subjected to hierarchical clustering (UPGMA, Spotfire) and correlation as a similarity measure. Horizontal rows represent individual genes; vertical columns represent individual samples. Black indicates average signal intensity, brightest red ≥ 4-fold up-regualtion, brightest green ≥ 4-fold down-regulated gene expression relative to the mean. Only probesets receiving P detection calls in 6 or more samples and a stander deviation of normalized signals > 0.2 were considered. (7239 retained). The dendogram at the top of the matrix indicates the degree of similarity between tumor samples (the higher the dendogramm, the lower the similarity). Two major clusters were identified as indicated. MR: Myxoid/Roundcell Liposarcoma, PL: Pleomorphic Liposarcoma, AL: Atypical Lipoma, ML: Myxoid Liposarcoma, DL: Dedifferentiated Liposarcoma, low: low responder group, medium: medium responder group, high: high responder group.