Figure 13

Effects of anti-sense P2X ₇ oligonucleotides on BzATP-induced increase in cytosolic calcium (ΔCa ²⁺ _i , A) and on the influx of ethidium bromide (Eth-Br, B). Cultured mouse wild-type normal keratinocytes were pre-treated with 100 μM anti-sense P2X₇ oligonucleotides (ASO) or random-control P2X₇ oligonucleotides (RCO) for 14 hours followed by 8 hours treatment with 100 μM BzATP. Control – cells treated with the vehicle of the ASO. Levels of ΔCa²⁺ _i (A) and of Eth-Br fluorescence (B) were determined as in Fig. 12. The experiments were repeated twice with similar trends.