Figure 5
From: Bone sialoprotein does not interact with pro-gelatinase A (MMP-2) or mediate MMP-2 activation
ProMMP-2 activity after incubation with BSP. ProMMP-2 (1.4 or 2.8 nM) was incubated with recombinant BSP (rBSP) or native BSP (nBSP) (4.9 or 9.8 nM) and 12.5 μg/mL fluorescent substrate. Results are values calibrated with fluorescence from substrate + BSP controls. Fluorescence levels of other controls, including substrate only, substrate + proMMP-2 and substrate + APMA-activated enzyme, are also shown.