Figure 6
From: Bone sialoprotein does not interact with pro-gelatinase A (MMP-2) or mediate MMP-2 activation
ProMMP-2 recovery in BSP-unbound sample. ProMMP-2 (0.5 mM) was added to decreasing concentrations of indicated SIBLING proteins (40 mM, 20 mM, 10 mM, 5 mM, 2.5 mM, 1.2 mM, 0.6 mM, and 0 mM) coated on an ELISA plate, and incubated at 21°C for 4 hours. Samples of the unbound (Supernatant) and bound (Extract) proteins were extracted in SDS sample buffer, and analyzed by zymography. proMMP-2 was recovered completely in the latent form in the unbound (Supernatant) fractions.