Figure 5
From: Groucho binds two conserved regions of LEF-1 for HDAC-dependent repression
Groucho and AES recruit HDAC for transcription repression. LEF-1•β-catenin activated TOPFlash was repressed by Groucho or AES (1000 ng) via transient transfection in COS-1 cells. Treatment with the HDAC inhibitor trichostatin A (50 ng/ml) relieved repression. Data is reported as described in Figure 3. Three independent experiments are shown. The error bars represent the spread of the duplicates for each experiment.