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Table 1 Oligonucleotides used for site-directed mutagenesis and electrophoretic mobility shift assay (EMSA)

From: Identification of target genes of transcription factor activator protein 2 gamma in breast cancer cells

Name

Sequence 5'-3'

Name pTAL-Luc vector

WtR3-36/HPSE(F)

GGTACCGGCCTGGACTGCAGACCT

 

WtR3-36/HPSE(R)

CTCGAGGCATGCCTGCAGGTCAAA

pTAL-R3-36/HPSE

WtR4-28/CDH2(F)

GGTACCCTGCGATGGGGGAGAACA

 

WtR4-28/CDH2(R)

CTCGAGTCCCCCCACGAGAAAAAA

pTAL-R4-28/CDH2

WtR1-15/IGSF11(F)

GGTACCTTCTGTCATTTTGGGTCT

 

WtR1-15/IGSF11(R)

CTCGAGTGGTTTTATCTGTGTAAT

pTAL-R1-15/IGSF11

WtR3-1/ErbB2 (F)

GGTACCGTAAAACGACGGCCAG

 

WtR3-1/ErbB2 (R)

CTCGAGAGGAAACAGCTATGAC

pTAL-R3-1/ErbB2

⋆WtR3-36-648/HPSE(F)

AAAAGTGCCCAGAGCCCATGA

 

⋆MtR3-36-648/HPSE(F)

AAAAGTattCAGgGCCCATGA

pTAL-R3-36-648/HPSE

⋆WtR3-36-676/HPSE(F)

AATGTGGCCCTGGTCACTTAG

 

⋆MtR3-36-676/HPSE(F)

AATGTGattCTGaTCACTTAG

pTAL-R3-36-676/HPSE

⋆WtR4-28-854/CDH2(F)

ATGCGACCTGCAGGCATGCCA

 

⋆MtR4-28-854/CDH2(F)

ATGCGACCcGCAaatATGCCA

pTAL-R4-28-854/CDH2

⋆WtR1-15-286/IGSF11(F)

TTTGCTGCCTTTGTCCTGTAG

 

⋆MtR1-15-286/IGSF11(F)

TTTGCTattTTTaTCCTGTAG

pTAL-R1-15-286/IGSF11

⋆HMT

AACGGACCGCCCGCGGCCCGT

 
  1. Note: ⋆ Only sense strands for oligonucleotides used in EMSA were shown; underlined parts were the putative binding sites, and mutated nucleotides were denoted as the lowercase letters. (F): forward primer; (R): reverse primer.
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BMC Cancer

ISSN: 1471-2407

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