Figure 1

DRB induces apoptotic death in T cell lines, LCLs and circulating lymphocytes. (A) DRB induces lymphocytes death. Cultured T cells from four donors (left), LCLs from three donors (middle) and circulating lymphocytes from three donors (right) were kept in medium alone or containing 10–100 μM DRB, harvested at 24, 48 and 72 hr and analysed by flow cytometry after stainig with PI. (B) DRB treatment induces translocation of phosphatidylserine to the external leaflet of the cell membrane. Cultured T cells from two donors (black bars), circulating lymphocytes from two donors (white bars) and LCLs from two donors (grey bars) were kept for 24 hr in medium alone or containing 10–100 μM DRB, harvested and stained with annexin V and PI. Percentages of PI- annexin V+ cells are indicated (mean ± S.D.). (C) DRB treatment induces caspase activation. Cultured T cells from four donors (black bars) and circulating lymphocytes from three donors (white bars) were kept for 24 hr in medium alone or containing 10–100 μM DRB, harvested and stained with anti-active caspase-3 antibody. Histograms show the percentage of active caspase-3⁺ cells (mean ± S.D.).