Figure 3
From: Vasohibin inhibits angiogenic sprouting in vitro and supports vascular maturation processes in vivo
Effects of adenoviral overexpression of vasohibins on cell proliferation and sprouting of endothelial cells. (A) Dot Blot analysis of VASH1 protein expression 48 h after adenoviral transfection of HUVECs. The used MOI was 100 virus/cell. A monoclonal antibody directed against the FLAG-tag (FLAG) was used to detect FLAG proteins. Tubulin alpha (Tub) served as internal loading control. Note: the polyclonal antibody directed against human VASH1 (hVASH1) does not recognize murine VASH1. (B) Cell proliferation was analyzed after adenoviral overexpression of human and human vasohibins or GFP. BrDU incorporation was measured 48 h post transfection. (C) HUVEC spheroids were stimulated with bFGF to induce angiogenic sprouting two days after adenoviral transfection. CSL indicates cumulative sprout length of the spheroid. GFP was used as control for unspecific adenoviral effects. (D) Angiogenic sprouting induced by the growth factor VEGF was significantly inhibited by VASH1 overexpression. Stars indicate p values < 0.05.