Rapid detection of SMARCB1 sequence variation using high resolution melting

BMC Cancer

Table 1 PCR conditions for SMARCB1 mutation screening

Exon	Primer	Amplicon size bp	Annealing temperature
5' Exon 1-1	INI1F actgagggcggcctggtcgt Snf1Rb ccgaaggtcttgctcagcgc	222	65°C
3' Exon 1-2	Snf1Fb ctctgccgccgcaatgatgatg INI1R cgacacgcccactaggccac	261	64°C
Exon 2	INI12F ctgcgacccttataatgagc INI12R gcgagtggttttgaaacagg	213	58°C
Exon 3	INI13F accagcagagtgacccagtg INI13R agagatgccctggccaggaa	195	60°C
Exon 4	INI14F tcgagcctgacagaggtacagtg INI14R gaatcagcacggagggtgagt	284	60°C
Exon 5	INI15F ttgcatacctagggctccgg INI15R cacgtaacacacaggggttg	238	60°C
Exon 6	INI16F tggtgcaatctcttggcatc INI16R tcagtgctccatgatgacac	277	60°C
Exon 7	INI17F tgggctgcaaaagctctaac INI17R cgctcacacagagaagtctt	312	60°C
Exon 8	INI18F atccactgggtgccagcagt INI18R tctgcctggaaagccaggtg	313	60°C
Exon 9	INI19F ccctgtagagccttgggaag INI19R gcctctgtccttgccagaag	200	60°C

ISSN: 1471-2407