Figure 1From: Liposome encapsulated zoledronate favours M1-like behaviour in murine macrophages cultured with soluble factors from breast cancer cellsDifferent mRNA and secreted cytokine profiles of 4T1 and 3T3 cells lead to differences in the modulation of J774 cell activation status with or without LPS stimulus. A mRNA expression levels of iNOS, IL-6, IL-12, and MMP-9. B Protein levels of M-CSF, VEGF, TNF-α, IL-10, MCP-1 and IL-6 secreted by macrophages. C Nitrite (NO2-) production. Data represent the means ± SEM of 5 independent experiments. *p < 0.05, one-way ANOVA with Dunnett’s multiple comparison test as compared with the CTR. D Zymography analysis of pro-MMP-9 in cell supernatants . Data represent the means ± SEM of four independent experiments. *p < 0.05, **p < 0.005, student’s t-test as compared with the CTR or the CTR + LPS treatment (* lines above the bars). E-G Data represent the means ± SEM of 3 independent 72-h post-confluence cultures assayed in duplicate. E Mean mRNA expression levels of iNOS, IL-6, IL-12, and MMP-9 normalised to GAPDH. F Secreted protein levels of M-CSF, VEGF, TNF-α, IL-10, MCP-1 and IL-6. G Zymography analysis of pro-MMP-9 in cell supernatants.Back to article page