Figure 6From: Microfluidic model of ductal carcinoma in situ with 3D, organotypic structureSecond Harmonic Generation (SHG) imaging of collagen modifications by invasive clusters. Multiphoton image of a DCIS model cocultured with HMFs. Cells were stained with phalloidin to mark f-actin (shown in red). Using SHG imaging we are able to identify increased collagen I (show in white) modification near the invasive region compared to non-invasive regions. Scale bar represents 100 μm.Back to article page