Figure 3

NO enhances PVR/CD155 expression: molecular mechanisms. A) PVR/CD155 surface expression was analyzed by flow cytometry on SKO-007(J3) cells treated with DETA-NO (200 μM) in the presence or absence of the guanylate cyclase inhibitor ODQ (50 μM) for 48 h. Data are representative of one out of three independent experiments. B) Western Blot analysis of total cellular proteins from SKO-007(J3) cells treated with DETA-NO for 18 h. The arrow indicates the expression of the pH2A.X and β-actin, used as loading control. The proteins transferred to nitrocellulose membranes were stained with Ponceau to verify that similar amounts of protein had been loaded in each lane. Data shown are representative of 1 out of 2 independent experiments. C) Western Blot analysis of total cellular proteins from SKO-007(J3) cells treated with DETA-NO for 18 h. Lysates were probed with antibodies to different phosphorylation sites of Chk1 and Chk2, wt Chk1 and Chk2 or β-actin, used as loading control. The proteins transferred to nitrocellulose membranes were stained with Ponceau to verify that similar amounts of protein had been loaded in each lane. Data shown are representative of 1 out of 2 independent experiments.