Figure 6

Regulation of PVR/CD155 expression on MM cell lines following treatment with the NO-releasing prodrugs NitroAspirin and JS-K. A) PVR/CD155 surface expression was analyzed by flow cytometry on SKO-007(J3) cells treated with NCX4040 (10 μM) for 48 h. Data are representative of one out of three independent experiments. The grey colored histogram represents basal expression of the indicated ligand, while thick black colored histogram represents the expression after treatment with NCX4040. B) The MFI of PVR/CD155 surface expression was calculated based on at least three independent experiments and evaluated by paired Student t test (*P < 0.05). Histograms represent the MFI with specific mAb subtracted from the MFI value of isotype control. C) Molecular structure of NCX4040. D) PVR/CD155 surface expression was analyzed by flow cytometry on SKO-007(J3) cells treated with JS-K (3 μM) for 48 h. Data are representative of one out of three independent experiments. The grey colored histogram represents basal expression of the indicated ligand, while thick black colored histogram represents the expression after treatment with JS-K. E) The MFI of PVR/CD155 surface expression was calculated based on at least three independent experiments and evaluated by paired Student t test (*P < 0.05). Histogram represents the MFI with specific mAb subtracted from the MFI value of isotype control. F) Molecular structure of JS-K. The concentration of the indicated donor used in these experiments, did not significantly affect cell viability as assessed by PI staining (data not shown).