Figure 5From: Low levels of IGFBP7 expression in high-grade serous ovarian carcinoma is associated with patient outcomeEffects of IGFBP7 recombinant protein on EOC cell lines. A, Western blot analysis showing an alteration of the phophorylation status of ERK and AKT proteins after rIGFBP7 treatment of 1 ng/μl of OV90 neor cell line as determined at different time points following exposure. Total AKT and total ERK served as a loading control. B, Spheroid formation assay with OV90 neor and TOV112D cell lines in the presence or absence of rIGFBP7 (1 and 10 ng/μl). Images are shown at ×20 magnification. C, wound healing assay of OV90 neor and TOV112D in the presence or absence of rIGFBP7 (1 and 5 ng/μl) observed over a period of 6 days. Images are shown at × 10 magnification.Back to article page