Figure 1

Characterization of HSD10-transfected cell lines. A. Empty vector (EV) and HSD10 overexpression (HSD10 ov) whole cell lysates were analyzed for HSD10 protein expression via immunoblotting. β-actin was used as the loading control, and HSD10 expression was normalized to actin (n = 4). B. Confocal microscopy demonstrating immunofluorescence staining of HSD10 alone (green), Mito Tracker Red alone (red), and these two antigens co-localized (yellow) in EV and HSD10 ov cells. Scale bar in B: 30 μm. C. Quantification of HSD10 immunofluorescence staining (depicted in B) displayed as fold increase (n = 5). D. Control shRNA and HSD10 shRNA whole cell lysates were analyzed for HSD10 protein expression via immunoblotting. β-actin was used as the loading control, and HSD10 expression was normalized to actin (n = 4). E. Confocal microscopy demonstrating immunofluorescence staining of HSD10 alone (green), Mito Tracker Red alone (red), and these two antigens co-localized (yellow) in control shRNA and HSD10 shRNA cells. Scale bar in E: 30 μm. F. Quantification of HSD10 immunofluorescence staining (depicted in E) displayed as fold increase (n = 5). Data presented as mean ± SE. *P < 0.01, **P < 0.001, ***P < 0.0001 verses control group.