Figure 4

Xenograft assay of the CAF. A. Tissue mass in the liver and lung of the NOD/SCID mouse following orthotopic injection of the CAF from HCC. Arrow indicates nodules. B. Primary cells of the nodules in the orthotopic xenografts. Scale bar, 100 μm. C. The positivity of genes in the xenograft primary cells. H = hepatic, L = lung, C + H = positive control human (human liver), C + M = positive control mouse (mouse liver). Gene 18S is homolog for human and mouse species. D. DNA sequencing of human AFP of primary cells obtained from the lung of xenograft. REF_AFP = DNA sequences of AFP genes from injected CAF, XG_AFP = DNA sequences of AFP genes from primary cultures of xenograft, NM_001134.1 = reference DNA sequences from GenBank.