Figure 3

NPC1L1 knockout decreasing plasma lipid and reducing inflammatory sensitivity may be involved in the mechanism.(A) Western blot. Total proteins from small intestines and membrane proteins from adjacent colon mucous membranes or tumors were blotted. NPC1L1 in mice colorectal mucous membranes or colorectal tumors was undetectable by western blot. Membrane protein loading control α-tubulin and membrane protein E-cadherin were easily detected in tumors or adjacent colorectal mucous membranes. N-cadherin was easily detected in small intestine tissues while it was undetectable in tumors or adjacent colorectal mucous membranes. (B) Plasma samples from 12 W old mice without any treatment (control), 10 W or 20 W after AOM injection were assayed for FC, TC, TG and PL. *p < 0.05, **p < 0.01. (C) The intestinal inflammation scores of NPC1L1−/− mice were significantly lower than those of WT mice. *p < 0.05. (D) Western blot. NPC1L1 ablation decreased inflammatory markers p-c-Jun, p-ERK and Caspase-1 p20 in tumors. (E) Fresh enlarged (sciatic and lumbar) lymph nodes. Mice were sacrificed 20 weeks after AOM injection. (F) H&E staining of enlarged lymph nodes showed that metastasis was not found. (Scale bars represent 400 μm).