Figure 6

Platelets increase Tissue Factor (TF) levels. A: Levels of mRNA relative to control of ovarian cancer cell lines SKOV3 and UCI101 co-cultured with platelets for 6 hours (N = 3). B and C: Western blot analysis of cell lines SKOV3 and UCI101 co-cultured with platelets during 12 or 24 hours. Above: density analysis of western blot bands respect to H3 levels. (N = 3). D: Flow cytometry analysis of TF levels of ovarian cancer spheroid of UCI101 cells formed in presence or absence of platelets (N = 3) E: Procoagulant activity assay (units of FXa formed) of ovarian cancer cell lines cultured during 24 h with or without platelets (N = 3). *p < 0.05; **p < 0.01; ***p < 0.001 respect to control, student´s t-test.