Figure 8

NF-κB DNA binding activity in DHMEQ 3 μg/ml, paclitaxel 10nM, or their combination treatment. Proteins were separately extracted from the cytoplasm and nucleus of each treated T24PR cells after the treatment of DHMEQ 3 μg/ml, paclitaxel 10nM, or their combination. 2 μl nuclear extract (at a concentration of 2 μg/μl) mixed with biotin-labeled NF-κB probe for EMSA assay was used.