Figure 3

Co-treatment of TH-302 and AZD7762 increased tail moment but not cross-linking by Comet assay. After seeding HT-29 cells for 24 h, cells were treated at the indicated drugs and oxygen concentrations for 24 h. To assess DNA breaks, single cell gel electrophoresis comet assay was performed (A). The length of tail moment (TM) was calculated using Comet Assay IV software (B). For cross-linking assessment, cells were treated with 20 μM of bleomycin for 1 h at the end of the drug treatment, and then Comet assay was performed (C) and TM was calculated (D). The data are the representative of three independent experiments.