Fig. 12From: NBPF1, a tumor suppressor candidate in neuroblastoma, exerts growth inhibitory effects by inducing a G1 cell cycle arrestNBPF1 overexpression induces no nuclear accumulation of p53 in NB cell lines. SH-SY5Y, NLF and SK-N-AS cells were transfected with a plasmid encoding either EGFP-luciferase (top panel) or EGFP-NBPF1 (lower panel). Immunofluorescence for p53 revealed no increased accumulation in the nucleus upon EGFP-NBPF1 transfection in either cell line. EGFP-luciferase transfected cells were stained with an anti-EGFP antibody, whereas EGFP-NBPF1 transfected cells were stained with the anti-NBPF antibody sc-82241, both represented in green. Nuclei were counterstained with DAPI. Scale bars: 10 μmBack to article page