Fig. 2

Time-lapse microscopy of NBPF1 expression in transfected HEK293T cells. Expression of EGFP-luciferase or EGFP-NBPF1 was followed for 15 h with images captured every 10 min. Individual frames are shown. Original movies are available as Additional files 1 and 2. Fluorescence levels are represented by pseudocolors, where blue indicates low EGFP levels and yellow indicates high EGFP levels (ImageJ LUT; color codes shown at the bottom). (A) Expression of EGFP-luciferase in cells remained at a constant level at all the indicated time points and did not interfere with cell division or induce cell death. The arrow points to an EGFP-positive mitotically dividing cell. (B) Expression of EGFP-NBPF1 in cells fluctuated, with some cells showing decreasing levels and others showing delayed expression. In no case were EGFP-NPBP1 expressing mitotic cells observed