Fig. 9From: NBPF1, a tumor suppressor candidate in neuroblastoma, exerts growth inhibitory effects by inducing a G1 cell cycle arrestNBPF1 overexpression has no effect on the mRNA levels of the TP53 gene. HEK293T and HEK293_shRNAp53 cells were transfected with plasmids encoding either EGFP-luciferase or EGFP-NBPF1. Both EGFP-positive (+) and EGFP-negative populations (−) were isolated by FACS and the expression levels of TP53 were determined by real-time quantitative RT-PCRBack to article page