Fig. 1
From: miR-16 promotes the apoptosis of human cancer cells by targeting FEAT
Expression levels of the FEAT protein, FEAT mRNA, and miR-16 in cancer tissues. a Western blotting analysis of the expression levels of the FEAT protein in three pairs of lung (LCT), breast (BCT), and hepatocellular cancer tissues (HCT) and in normal adjacent tissues (NAT). b Quantitative RT-PCR analysis of the relative expression levels of FEAT mRNA in three pairs of lung, breast, and hepatocellular cancer tissues and in normal adjacent tissues. c Schematic description of the hypothetical duplexes formed by the interactions between the binding site in the FEAT 3′-UTR (top) and miR-16 (bottom). The predicted free energy value of the hybrid is indicated. The seed recognition site is denoted, and all nucleotides in this region are highly conserved across species. (D) Quantitative RT-PCR analysis of the expression levels of miR-16 (in the form of the miRNA/U6 ratio) in three pairs of lung, breast, and hepatocellular cancer tissues and in normal adjacent tissues. **, P < 0.01; ***, P < 0.001