Fig. 5

Constitutive Bcl-2 expression induces a migratory and invasive phenotype. a The motility behavior of control cells and Bcl-2 transfected cells was analyzed in an in vitro wound-healing assay. b Statistical significance (p < 0.01) exhibited for the Bcl-2 transfected cells with stronger migratory capability (N = 3; mean = 65.33; SE = 3.51) than the control ones (N = 3; mean = 11; SE = 2.0).. Data showed the counted cell numbers, and represented as mean ± s.e.m. c The invasive features of the control and Bcl-2 expressing cells were analyzed in an invasion assay on matrigel in a BioCoat Matrigel Invasion Chamber. The cells adhered to the lower surface of the filters were fixed and stained with a Diff-Quik Stain Set, photographed under a light microscope. d Statistical significance (p < 0.01) exhibited for the Bcl-2 transfected cells with stronger invasive potential (N = 3; mean = 57.67; SE = 4.51) than the control ones (N = 3; mean = 4.67; SE = 1.53)